• EDTA 10 gr merck

During the cell lysis step of DNA extraction, the cytoplasm and nucleoplasm are broken open. On leaving the nucleus, DNA will encounter enzymes called DNAses, as well as other nucleases, which disrupt the structure of the DNA. Many of these DNAses require Mg2+ ions as a cofactor. This is where the use of EDTA becomes important. EDTA binds Mg2+ ions, thus depriving nucleases of this essential co-factor. In this way, the use of EDTA prevents the DNA from being disrupted and increases the DNA yield during the extraction process